A Dual Motif in the Hemagglutinin of H5N1 Goose/Guangdong-Like Highly Pathogenic Avian Influenza Virus Strains Is Conserved from Their Early Evolution and Increases both Membrane Fusion pH and Virulence
Identifieur interne : 000230 ( Main/Exploration ); précédent : 000229; suivant : 000231A Dual Motif in the Hemagglutinin of H5N1 Goose/Guangdong-Like Highly Pathogenic Avian Influenza Virus Strains Is Conserved from Their Early Evolution and Increases both Membrane Fusion pH and Virulence
Auteurs : Ute Wessels [Allemagne] ; Elsayed M. Abdelwhab [Allemagne] ; Jutta Veits [Allemagne] ; Donata Hoffmann [Allemagne] ; Svenja Mamerow [Allemagne] ; Olga Stech [Allemagne] ; Jan Hellert [France] ; Martin Beer [Allemagne] ; Thomas C. Mettenleiter [Allemagne] ; Jürgen Stech [Allemagne]Source :
- Journal of Virology [ 0022-538X ] ; 2018.
Abstract
Zoonotic highly pathogenic avian influenza viruses (HPAIV) have raised serious public health concerns of a novel pandemic. Their prime virulence determinant is the polybasic hemagglutinin (HA) cleavage site. However, required coadaptations in the HA (and other genes) remained uncertain. Here, we identified the dual motif 123R/124I in the HA head that increases the activation pH of HA-mediated membrane fusion, essential for virus genome release into the cytoplasm. This motif is extremely predominant in H5 HPAIV and emerged already in the earliest 1997 H5N1 HPAIV. Reversion to 123S or 124T, characteristic of low-pathogenic strains, attenuated the virus in chicken and mice, accompanied by a decreased HA activation pH. This increased pH sensitivity of H5 HPAIV extends the viral tropism to cells with less-acidic endosomes, e.g., within the respiratory tract. Therefore, early HA adaptation to increased acid sensitivity promoted the emergence of H5 Goose/Guangdong-like HPAIV strains and is required for their zoonotic potential.
Url:
DOI: 10.1128/JVI.00778-18
PubMed: 29899102
PubMed Central: 6096801
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000706
- to stream Pmc, to step Curation: 000706
- to stream Pmc, to step Checkpoint: 000177
- to stream Ncbi, to step Merge: 000E84
- to stream Ncbi, to step Curation: 000E84
- to stream Ncbi, to step Checkpoint: 000E84
- to stream Main, to step Merge: 000230
- to stream Main, to step Curation: 000230
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A Dual Motif in the Hemagglutinin of H5N1 Goose/Guangdong-Like Highly Pathogenic Avian Influenza Virus Strains Is Conserved from Their Early Evolution and Increases both Membrane Fusion pH and Virulence</title><author><name sortKey="Wessels, Ute" sort="Wessels, Ute" uniqKey="Wessels U" first="Ute" last="Wessels">Ute Wessels</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute of Molecular Virology and Cell Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Molecular Virology and Cell Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems</wicri:regionArea><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion></affiliation></author><author><name sortKey="Abdelwhab, Elsayed M" sort="Abdelwhab, Elsayed M" uniqKey="Abdelwhab E" first="Elsayed M." last="Abdelwhab">Elsayed M. Abdelwhab</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute of Molecular Virology and Cell Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Molecular Virology and Cell Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems</wicri:regionArea><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion></affiliation></author><author><name sortKey="Veits, Jutta" sort="Veits, Jutta" uniqKey="Veits J" first="Jutta" last="Veits">Jutta Veits</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute of Molecular Virology and Cell Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Molecular Virology and Cell Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems</wicri:regionArea><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion></affiliation></author><author><name sortKey="Hoffmann, Donata" sort="Hoffmann, Donata" uniqKey="Hoffmann D" first="Donata" last="Hoffmann">Donata Hoffmann</name><affiliation wicri:level="1"><nlm:aff id="aff2">Institute of Diagnostic Virology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Diagnostic Virology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems</wicri:regionArea><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion></affiliation></author><author><name sortKey="Mamerow, Svenja" sort="Mamerow, Svenja" uniqKey="Mamerow S" first="Svenja" last="Mamerow">Svenja Mamerow</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute of Molecular Virology and Cell Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Molecular Virology and Cell Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems</wicri:regionArea><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion></affiliation></author><author><name sortKey="Stech, Olga" sort="Stech, Olga" uniqKey="Stech O" first="Olga" last="Stech">Olga Stech</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute of Molecular Virology and Cell Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Molecular Virology and Cell Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems</wicri:regionArea><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion></affiliation></author><author><name sortKey="Hellert, Jan" sort="Hellert, Jan" uniqKey="Hellert J" first="Jan" last="Hellert">Jan Hellert</name><affiliation wicri:level="3"><nlm:aff id="aff3">Institut Pasteur, Département de Virologie, Unité de Virologie Structurale, Paris, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Institut Pasteur, Département de Virologie, Unité de Virologie Structurale, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Beer, Martin" sort="Beer, Martin" uniqKey="Beer M" first="Martin" last="Beer">Martin Beer</name><affiliation wicri:level="1"><nlm:aff id="aff2">Institute of Diagnostic Virology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Diagnostic Virology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems</wicri:regionArea><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion></affiliation></author><author><name sortKey="Mettenleiter, Thomas C" sort="Mettenleiter, Thomas C" uniqKey="Mettenleiter T" first="Thomas C." last="Mettenleiter">Thomas C. Mettenleiter</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute of Molecular Virology and Cell Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Molecular Virology and Cell Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems</wicri:regionArea><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion></affiliation></author><author><name sortKey="Stech, Jurgen" sort="Stech, Jurgen" uniqKey="Stech J" first="Jürgen" last="Stech">Jürgen Stech</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute of Molecular Virology and Cell Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Molecular Virology and Cell Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems</wicri:regionArea><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">29899102</idno><idno type="pmc">6096801</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6096801</idno><idno type="RBID">PMC:6096801</idno><idno type="doi">10.1128/JVI.00778-18</idno><date when="2018">2018</date><idno type="wicri:Area/Pmc/Corpus">000706</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000706</idno><idno type="wicri:Area/Pmc/Curation">000706</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000706</idno><idno type="wicri:Area/Pmc/Checkpoint">000177</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000177</idno><idno type="wicri:Area/Ncbi/Merge">000E84</idno><idno type="wicri:Area/Ncbi/Curation">000E84</idno><idno type="wicri:Area/Ncbi/Checkpoint">000E84</idno><idno type="wicri:doubleKey">0022-538X:2018:Wessels U:a:dual:motif</idno><idno type="wicri:Area/Main/Merge">000230</idno><idno type="wicri:Area/Main/Curation">000230</idno><idno type="wicri:Area/Main/Exploration">000230</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A Dual Motif in the Hemagglutinin of H5N1 Goose/Guangdong-Like Highly Pathogenic Avian Influenza Virus Strains Is Conserved from Their Early Evolution and Increases both Membrane Fusion pH and Virulence</title><author><name sortKey="Wessels, Ute" sort="Wessels, Ute" uniqKey="Wessels U" first="Ute" last="Wessels">Ute Wessels</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute of Molecular Virology and Cell Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Molecular Virology and Cell Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems</wicri:regionArea><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion></affiliation></author><author><name sortKey="Abdelwhab, Elsayed M" sort="Abdelwhab, Elsayed M" uniqKey="Abdelwhab E" first="Elsayed M." last="Abdelwhab">Elsayed M. Abdelwhab</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute of Molecular Virology and Cell Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Molecular Virology and Cell Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems</wicri:regionArea><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion></affiliation></author><author><name sortKey="Veits, Jutta" sort="Veits, Jutta" uniqKey="Veits J" first="Jutta" last="Veits">Jutta Veits</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute of Molecular Virology and Cell Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Molecular Virology and Cell Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems</wicri:regionArea><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion></affiliation></author><author><name sortKey="Hoffmann, Donata" sort="Hoffmann, Donata" uniqKey="Hoffmann D" first="Donata" last="Hoffmann">Donata Hoffmann</name><affiliation wicri:level="1"><nlm:aff id="aff2">Institute of Diagnostic Virology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Diagnostic Virology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems</wicri:regionArea><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion></affiliation></author><author><name sortKey="Mamerow, Svenja" sort="Mamerow, Svenja" uniqKey="Mamerow S" first="Svenja" last="Mamerow">Svenja Mamerow</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute of Molecular Virology and Cell Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Molecular Virology and Cell Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems</wicri:regionArea><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion></affiliation></author><author><name sortKey="Stech, Olga" sort="Stech, Olga" uniqKey="Stech O" first="Olga" last="Stech">Olga Stech</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute of Molecular Virology and Cell Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Molecular Virology and Cell Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems</wicri:regionArea><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion></affiliation></author><author><name sortKey="Hellert, Jan" sort="Hellert, Jan" uniqKey="Hellert J" first="Jan" last="Hellert">Jan Hellert</name><affiliation wicri:level="3"><nlm:aff id="aff3">Institut Pasteur, Département de Virologie, Unité de Virologie Structurale, Paris, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Institut Pasteur, Département de Virologie, Unité de Virologie Structurale, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Beer, Martin" sort="Beer, Martin" uniqKey="Beer M" first="Martin" last="Beer">Martin Beer</name><affiliation wicri:level="1"><nlm:aff id="aff2">Institute of Diagnostic Virology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Diagnostic Virology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems</wicri:regionArea><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion></affiliation></author><author><name sortKey="Mettenleiter, Thomas C" sort="Mettenleiter, Thomas C" uniqKey="Mettenleiter T" first="Thomas C." last="Mettenleiter">Thomas C. Mettenleiter</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute of Molecular Virology and Cell Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Molecular Virology and Cell Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems</wicri:regionArea><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion></affiliation></author><author><name sortKey="Stech, Jurgen" sort="Stech, Jurgen" uniqKey="Stech J" first="Jürgen" last="Stech">Jürgen Stech</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute of Molecular Virology and Cell Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Molecular Virology and Cell Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems</wicri:regionArea><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion><wicri:noRegion>Greifswald-Insel Riems</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of Virology</title><idno type="ISSN">0022-538X</idno><idno type="eISSN">1098-5514</idno><imprint><date when="2018">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Zoonotic highly pathogenic avian influenza viruses (HPAIV) have raised serious public health concerns of a novel pandemic. Their prime virulence determinant is the polybasic hemagglutinin (HA) cleavage site. However, required coadaptations in the HA (and other genes) remained uncertain. Here, we identified the dual motif 123R/124I in the HA head that increases the activation pH of HA-mediated membrane fusion, essential for virus genome release into the cytoplasm. This motif is extremely predominant in H5 HPAIV and emerged already in the earliest 1997 H5N1 HPAIV. Reversion to 123S or 124T, characteristic of low-pathogenic strains, attenuated the virus in chicken and mice, accompanied by a decreased HA activation pH. This increased pH sensitivity of H5 HPAIV extends the viral tropism to cells with less-acidic endosomes, e.g., within the respiratory tract. Therefore, early HA adaptation to increased acid sensitivity promoted the emergence of H5 Goose/Guangdong-like HPAIV strains and is required for their zoonotic potential.</p></div></front></TEI><affiliations><list><country><li>Allemagne</li><li>France</li></country><region><li>Île-de-France</li></region><settlement><li>Paris</li></settlement></list><tree><country name="Allemagne"><noRegion><name sortKey="Wessels, Ute" sort="Wessels, Ute" uniqKey="Wessels U" first="Ute" last="Wessels">Ute Wessels</name></noRegion><name sortKey="Abdelwhab, Elsayed M" sort="Abdelwhab, Elsayed M" uniqKey="Abdelwhab E" first="Elsayed M." last="Abdelwhab">Elsayed M. Abdelwhab</name><name sortKey="Beer, Martin" sort="Beer, Martin" uniqKey="Beer M" first="Martin" last="Beer">Martin Beer</name><name sortKey="Hoffmann, Donata" sort="Hoffmann, Donata" uniqKey="Hoffmann D" first="Donata" last="Hoffmann">Donata Hoffmann</name><name sortKey="Mamerow, Svenja" sort="Mamerow, Svenja" uniqKey="Mamerow S" first="Svenja" last="Mamerow">Svenja Mamerow</name><name sortKey="Mettenleiter, Thomas C" sort="Mettenleiter, Thomas C" uniqKey="Mettenleiter T" first="Thomas C." last="Mettenleiter">Thomas C. Mettenleiter</name><name sortKey="Stech, Jurgen" sort="Stech, Jurgen" uniqKey="Stech J" first="Jürgen" last="Stech">Jürgen Stech</name><name sortKey="Stech, Olga" sort="Stech, Olga" uniqKey="Stech O" first="Olga" last="Stech">Olga Stech</name><name sortKey="Veits, Jutta" sort="Veits, Jutta" uniqKey="Veits J" first="Jutta" last="Veits">Jutta Veits</name></country><country name="France"><region name="Île-de-France"><name sortKey="Hellert, Jan" sort="Hellert, Jan" uniqKey="Hellert J" first="Jan" last="Hellert">Jan Hellert</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000230 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000230 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:6096801
|texte= A Dual Motif in the Hemagglutinin of H5N1 Goose/Guangdong-Like Highly Pathogenic Avian Influenza Virus Strains Is Conserved from Their Early Evolution and Increases both Membrane Fusion pH and Virulence
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:29899102" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a H2N2V1
| This area was generated with Dilib version V0.6.33. |  |